Studies on Sulfhydryl Groups during Cell Division of Sea Urchin Egg

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Studies on Sulfhydryl Groups during Cell Division of Sea Urchin Egg

The contractility of the thread model prepared from the KCl-soluble proteins of the egg and in vivo factors for the contraction are investigated in Hemicentrotus, Anthocidaris, and Pseudocentrotus eggs. The contractility of the thread model induced by metal ions or cystine changes during development in the characteristic pattern of high at the metaphase and low at the monaster and the interkine...

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Studies on Sulfhydryl Groups during Cell Division of Sea Urchin Egg V. Change in contractility of the thread model in relation to cell division

The contractility of the thread model prepared from the KCIsoluble proteins of the egg and in vivo factors for the contraction are investigated in Hemicentrotus, Anthoddaris, and Pseudocentrotus eggs. The contractility of the thread model induced by metal ions or cystine changes during development in the characteristic pattern of high at the metaphase and low at the monaster and the interkineti...

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STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG III. - -SH Groups of KC1-Soluble Proteins and Their Change during Cleavage

Sea urchin egg proteins extracted with KC1 are mostly TCA-soluble and, conversely, those extracted with T CA are KCl-soluble. Both groups are water-insoluble and show fluctuations in S H content dur ing the division cycle. The fluctuation of the S H groups of the KC1soluble protein of the whole egg is due to a S H ~ S S in terchange within the freely react ing groups and not within the sluggish...

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STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG II. Mass Isolation of the Egg Cortex and Change ill I t s - -SH Groups during Cell Division

Masses of cortices of both unfertilized and fertilized sea urchin eggs can be isolated by crushing eggs in hypotonic MaCI~ (0.1 M) solution. The amount of cortical material in terms of protein-N increases steadily after fertilization until the monaster stage and thereafter remains almost constant until well into the two-cell stage. The amount of bound S H per protein-N of the egg cortex also in...

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Calcium in sea urchin egg during fertilization.

Calcium plays a strikingly important role in two of the major events in developmental biology: cell activation and differentiation. In this review we begin with the location and quantity of intracellular calcium in sea urchin oocytes, and then discuss the changes that occur during fertilization and egg activation, placing special emphasis on the mobilization and redistribution of intracellular ...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1962

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.45.3.411